RiboToolkit | RiboAlign

Library alignment

Upload your customized library to profile RPF expression:

Input should be job IDs and the library that is in FASTA format.
To speed the uploading, we highly recommend the users to compress the file into .zip or .gz format.

The fields marked by "*" are required!

Library *

Human (.fa, .fa.zip, .fa.gz)

job ID(s) *

Provide completed job ID(s) to analyze your library (Example).

Length of interval (bp) *

Shortest tag Length Largest tag Length

Alignment tool

Bowtie MegaBLAST

Alignment type

Bowtie seed alignment Full end-to-end alignment

Allowed mismatch in seed region *

Mismatch 0-3

Bowtie seed length *

Mismatch 0-3

Allowed mismatchs in whole tag *

All mismatch in end-to-end mapping 0-10

Maximum of tag mapping times *

Default mapping time threshold for multiple mapping

MEGABLAST Parameters

E value

B value

V value

Get an notification when the job is done (optional)