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Upload your small RNAs Next-Generation Sequencing data to detect and profile sncRNA modifications

  • Input files could be in collapsed FASTA format
  • To reduce the file size, we highly recommend that the file is further compressed in .zip or .gz format
  • References:
    1. Nucleotide resolution profiling of m7G tRNA modification by TRAC-Seq. Nature Protocol. 2019
    2. Mettl1/Wdr4-Mediated m7G tRNA Methylome Is Required for Normal mRNA Translation and Embryonic Stem Cell Self-Renewal and Differentiation. Molecular Cell. 2018 Jul 19

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Upload samples and select the reference genome

Please choose a species for the analysis


Upload sequences/GSE sample ID

You can upload collapsed FASTA file (.fa, .zip, .gz)

You can upload collapsed FASTA file (.fa, .zip, .gz)

Parameters for sncRNA modifications identification

Mismatch 0-3
multiple-mapping times
Minimum of cleavage score
cleavage ratio threshold
P-value
When the job is complete, you can get an notification (optional field)