sncRNA study  

Upload your small RNAs Next-Generation Sequencing data to detect and profile sncRNAs:

  • Input files could be in collapsed FASTA (Recommend) (with Fasta head like >A_xB,where A is unique seq name while B is the frequency of A), FASTQ, GSE sample ID and web link to collapsed FASTA file
  • To speed the uploading, we highly recommend that the upload file is further compressed in .zip or .gz format

The fields marked by "*" are required!

Upload samples and select the reference genome

Please choose a species for the analysis

Select file Change Remove
Please upload sequences files 
(Hsa)    (Mmu)   (Populus)
When the job is complete, you can get an notification (optional field)

Common parameters

Shortest tag Length Largest tag Length
Mismatch 0-3
Default mapping time threshold for multiple mapping

miRNA analysis parameters

Flank sequence length of the query. Range 0 - 200
Maximum of mapping times for for miRNA

tRFs analysis parameters

Min. tag abundance (Read Rer Million)
P-value inferred based on Binomial statistics method to distinguish random fragments
m: Maximum of mapping times for tRNA&tRF

piRNA/24nt siRNA analysis parameters

Minimum read depth of 24-nt siRNAs to call clusters.
The neighbor clusters with distance smaller than this value will be merged. With step: 50
Maximum of mapping times for piRNA/24nt siRNA tags

PhasiRNA analysis parameters

Periodicitic length of phased siRNAs (bp)
Minimum read depth for p-value computations.