Batch sncRNA identification  

Upload your small RNAs Next-Generation Sequencing data to detect and profile sncRNAs:

  • Inputs could be collapsed FASTA files (Recommend) (with Fasta head as >A_xB,where A is unique seq name while B is the frequency of A), FASTQ format, GSM/SRR sample IDs or accessible links to FASTA files
  • To speed the uploading, we highly recommend that the upload file is further compressed in .zip or .gz format

The fields marked by "*" are required!

Upload samples and select the reference genome

Please choose a species for the analysis

Fasta: (Hsa)  (Mmu)   (Populus)
Cleaned fastq: hsa (trimmed)  ath (trimmed)
Raw fastq: hsa (raw)  ath (raw)

Separate multiple names by comma ","
Get an notification when the job is done (optional)

Common parameters

Shortest tag Length Largest tag Length
Mismatch 0-3
Mismatch 0-3
Default mapping time threshold for multiple mapping

Novel miRNA analysis parameters

The probability that the hairpin is a genuine miRNA precursor

tRFs analysis parameters

Min. tag abundance (Read Rer Million)
P-value inferred based on Binomial statistics method to distinguish random fragments
m: Maximum of mapping times for tRNA&tRF

piRNA/siRNA analysis parameters

Minimum tag depth to call clusters.
The neighbor clusters with distance smaller than this value will be merged. With step: 50
Maximum of mapping times for piRNA/siRNA tags

PhasiRNA analysis parameters

Periodicitic length of phased siRNAs (bp)
Minimum read depth for phasiRNA.