Nm modification, is a post-transcriptional chemical modification widely found in RNA molecules. This modification involves the addition of a methyl group (-CH3) to the ribose 2'-OH position of the RNA molecule. This modification is prevalent in a wide range of RNA species, including mRNAs, non-coding RNAs (ncRNAs), transfer RNAs (tRNAs), ribosomal RNAs (rRNAs), and other types of RNAs. nm modification not only enhances the structural integrity of RNAs, but also confers unique structural and functional properties to modified RNA molecules, such as facilitating the formation of additional hydrogen bonds, thereby affecting processes such as RNA stability, localization, splicing, and translation. Here, we present the “Nm-Atlas”, a carefully compiled database for the comprehensive collection and annotation of ribosylmethylation modifications in RNA.

The sequencing methodologies employed comprise second-generation techniques such as RiboMeth-seq, as well as third-generation sequencing methods like Direct RNA sequencing (DRS). The Nm-Atlas offers an intuitive interface for information display and a myriad of analytical tools, including RMlevelDiff, NmVar, RNAmodNet, and Blast (for functional analysis). Additionally, it provides resources such as mRNAbrowse, RNAlollipop, JBrowse, and Integrative Genomics Viewer (for data visualization). In essence, these resources present unparalleled opportunities for exploring the epitranscriptomics of pseudouridine.

Nm-Atlas: an integrated database for RNA modifications

Please cite us with this Reference