Small non-coding RNAs (sRNA/sncRNAs) are generated from different genome locus and have shown that sncRNAs in fungi play crucial roles in various biological processes such as development, stress responses, virulence, and pathogenicity. Understanding the functions and regulatory mechanisms of these small RNAs can provide valuable insights into fungal biology and potentially lead to the development of novel antifungal strategies.In fungi, sncRNAs can be broadly categorized into several classes based on their size and biogenesis mechanisms. Some of the well-studied classes of sncRNAs in fungi include:MicroRNAs (miRNAs): These are small RNAs typically around 21-24 nucleotides in length that are processed from hairpin precursor transcripts. MiRNAs in fungi regulate gene expression by base-pairing with target mRNAs, leading to mRNA degradation or translational inhibition; Small interfering RNAs (siRNAs): siRNAs are typically 20-25 nucleotides long and are generated from double-stranded RNA precursors. In fungi, siRNAs are involved in various processes including heterochromatin formation, transposon silencing, and antiviral defense; Piwi-interacting RNAs (piRNAs): Although more commonly studied in animals, piRNAs have also been identified in some fungi. These small RNAs, typically 24-30 nucleotides long, associate with Piwi proteins and play a role in transposon silencing and genome stability;Endogenous small interfering RNAs (endo-siRNAs): These sncRNAs are derived from endogenous loci and participate in gene regulation. Endo-siRNAs can target protein-coding genes or repetitive elements in the genome.

Next-generation sequencing (NGS) has provided an unprecedented opportunity to discover and quantify sRNAs and there are many tools have been developed to analysis sRNAs. However, to the best of our knowledge, most of available tools pay the main attention to study miRNA and related function. The comprehensive web-based tool to bench analysis of sRNAs in fungi and their potential function is still lacking.The highly repetitive elements content in these small RNAs impede the identification of sRNA modification based on conventional immunoprecipitation methods. In this study, a novel database FungiSNC is presented, which can be used to discovery, profile and functional annotate diverse kind of sRNAs. Beside, sRNA modifications can be identified with site resolution based on cleavage based technology.

FungiSNC have these main features:

• sRNA can be detected and profiled for as many as 29 species
• Unbiased classify the sRNA into different categories
• Differential expression analysis with group case small RNAs transcriptome
• Gene targets and function prediction for sRNA
• Diverse ways to upload small RNA sequencing data in very interactive queue system
• sRNA modification calling using cleavage-based method
• Local versions based on program package are also available
• Very user-friendly web interfaces and convenient data analysis queue system

FungiSNC: an integrated database for fungi small RNA

Please cite us with this Reference