FastQCFastQC Report
Tue 16 Jul 2019
human_SRR6442216.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filenamehuman_SRR6442216.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7177535
Sequences flagged as poor quality0
Sequence length152
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCTGGTCCGATGGTAGTGGGTTATCAGAACTAGATCGGAAGAGCACACG3110264.333326134947443No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCTAGATCGGAAGAGCACACGT1243181.7320431039347075No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCAGATCGGAAGAGCACACGT913751.2730693754889388No Hit
TGCTCTGATGAAATCACTAATAGGAAGTGCCGTCAGAAGCGATAACTGAC839361.1694265510373687No Hit
GTTTGTGATGACTTACATGGAATCTCGTTCGGCTGATGACTTGCTGTTGA756151.0534953852541298No Hit
TAGCTTATCAGACTGATGTTGACAGATCGGAAGAGCACACGTCTGAACTC735811.0251569654484443Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACATGTCAATCTCGTATG634370.8838271077744658TruSeq Adapter, Index 15 (97% over 49bp)
GGCTGGTCCGATGGTAGTGGGTTATCAGAACCAGATCGGAAGAGCACACG616180.858484145322872No Hit
TCTTTGGTTATCTAGCTGTATGAGATCGGAAGAGCACACGTCTGAACTCC597040.8318176086915633Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAAAGATCGGAAGAGCACA578530.8060288107268024No Hit
CCTGGATGATGATAAGCAAATGCTGACTGAACATGAAGGTCTTAATTAGC552270.7694424339275253No Hit
GTGAAATGATGGCAATCATCTTTCGGGACTGACCTGAAATGAAGAGAATA531550.7405745844499539No Hit
TCTTTGGTTATCTAGCTGTATGAAGATCGGAAGAGCACACGTCTGAACTC527910.7355032054876779Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
TAGCTTATCAGACTGATGTTGATAGATCGGAAGAGCACACGTCTGAACTC477880.6657996094759552Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
TGAGGTAGTAGATTGTATAGTTAGATCGGAAGAGCACACGTCTGAACTCC474210.6606864334343197Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
GGCTGGTCCGATGGTAGTGGGTTATCAGAACTTAGATCGGAAGAGCACAC440230.6133442748798856No Hit
TGAGGTAGTAGTTTGTGCTGTTAGATCGGAAGAGCACACGTCTGAACTCC422150.5881545683859432Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
AGTAGTGATGAAATTCCACTTCATTGGTCCGTGTTTCTGAACCACATGAT398520.5552324022105082No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCAAGATCGGAAGAGCACACGT368210.5130034196977096No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACAAGATCGGAAGAGCACACG354820.49434798994362267No Hit
CTCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAGATC352660.49133860022974457No Hit
TCCCACATGGTCTAGCGGTTAGGATTCCTGGTTAGATCGGAAGAGCACAC329390.4589179990066227No Hit
GGGGATGTAGCTCAGTGGAGATCGGAAGAGCACACGTCTGAACTCCAGTC297730.41480814792265036Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
GGCTGGTCCGATGGTAGTGGGTTATCAGAACAGATCGGAAGAGCACACGT285100.3972115775123354No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCAGATCGGAAGAGCACACGTC278910.3885874468045088No Hit
ATACATGATGATCTCAATCCAACTTGAACTCTCTCACTGATTACTTGATG277190.3861910809212355No Hit
TGAGGTAGTAGTTTGTACAGTTAGATCGGAAGAGCACACGTCTGAACTCC269670.37571394636180805Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TGTAAACATCCTCGACTGGAAGCTAGATCGGAAGAGCACACGTCTGAACT253710.3534779001425977Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
CGCGACCTCAGATCAGACGTGGCGACCCGTAGATCGGAAGAGCACACGTC252600.3519314082062992No Hit
GCATTGTGGTTCAGTGGTAGAATTCTCGCTAGATCGGAAGAGCACACGTC246270.34311222446146206No Hit
TTCAAGTAATCCAGGATAGGCTAGATCGGAAGAGCACACGTCTGAACTCC226260.31523357252873024Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
CACAGATGATGAACTTATTGACGGGCGGACAGAAACTGTGTGCTGATTGT196200.27335289901059345No Hit
CGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACGCAGC187270.2609113017212734No Hit
AAGCTATGATGAATTTGATTGCATTGATCGTCTGACATGATAATGTATTT184800.25747000885401466No Hit
TATCTGTGATGATCTTATCCCGAACCTGAACTTCTGTTGAAAAAAAAAAA180790.2518831325796391No Hit
GCATTGTGGTTCAGTGGTAGAATTCTCGCCAGATCGGAAGAGCACACGTC178420.24858116331024507No Hit
TGAAATGATGGCAATCATCTTTCGGGACTGACCTGAAATGAAGAGAATAC176220.2455160441572211No Hit
TCTTTGGTTATCTAGCTGTATAAGATCGGAAGAGCACACGTCTGAACTCC172270.24001276204156438Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
CTACGGGGATGATTTTACGAACTGAACTCTCTCTTTCTGATGGATTAGTG167510.23338095878320345No Hit
GGAATACCGGGTGCTGTAGGCTTTAGATCGGAAGAGCACACGTCTGAACT163740.22812845914370322Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
TGAGGTAGTAGGTTGTATAGTTAGATCGGAAGAGCACACGTCTGAACTCC159340.22199822083765527Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
GATGGGAGACCGCCTGGGAATACCGGGTGCTGTAGGCTTAGATCGGAAGA152370.21228736606648382No Hit
CTCACTGATGAGTACGTTCTGACTTTCGTTCTTCTGAGTTTGCTGAAGCC149210.20788474037395846No Hit
GGAGTACATTAGTCTTAATTGACTTATTACATAATCGATTCGTGTCTAGT145500.20271583489317713No Hit
GGGAGCGTAGCTCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACATG144410.2011972076764516TruSeq Adapter, Index 15 (100% over 36bp)
CTGCTGTGATGACATTCCAATTAAAGCACGTGTTAGACTGCTGACGCGGG139530.19439821610065294No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACGAGATCGGAAGAGCACACG134100.18683294473659828No Hit
GTGCAATGATGTATTTTATTCAACACATCATTCTGAAAGAACGTGTGGAA133300.18571835595368047No Hit
CTGGATGATGATAAGCAAATGCTGACTGAACATGAAGGTCTTAATTAGCT133160.18552330291666985No Hit
GCATGGGTGGTTCAGTGGTAGAATTCTCGCTAGATCGGAAGAGCACACGT132960.1852446557209404No Hit
TAGATATAGGAATAGAAAACTCTAGATCGGAAGAGCACACGTCTGAACTC131880.18373996086400135Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
TAGCTTATCAGACTGATGTTGACTAGATCGGAAGAGCACACGTCTGAACT130980.18248604848321884Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
TCTTTGGTTATCTAGCTGTATGTAGATCGGAAGAGCACACGTCTGAACTC126280.17593783938357668Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
AACCCGTAGATCCGATCTTGTAGATCGGAAGAGCACACGTCTGAACTCCA123200.1716466725693431Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TCGCGTGATGACATTCTCCGGAATCGCTGTACGGCCTTGATGAAAGCACA121530.16931996848500216No Hit
CTGCAGTGATGACTTTCTTAGGACACCTTTGGATTTACCGTGAAAATTAA120870.16840043273909497No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAATAGATCGGAAGAGCACACGT119200.16607372865475403No Hit
TGTAAACATCCCCGACTGGAAGCTAGATCGGAAGAGCACACGTCTGAACT118640.16529351650671156Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
GGCTGGTCCGAAGGTAGTGAGTTATCTCAATAGATCGGAAGAGCACACGT118590.1652238547077792No Hit
TAGATATAGGAATAGAAAACTCTTAGATCGGAAGAGCACACGTCTGAACT118530.16514026054906036Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
TCTTTGGTTATCTAGCTGTATAGATCGGAAGAGCACACGTCTGAACTCCA118280.16479195155439855Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TCCCATATGGTCTAGCGGTTAGGATTCCTGGTTAGATCGGAAGAGCACAC116490.16229805915261997No Hit
TTTGAATGATGACTTTAATTGTCGGATACCCCTTCACTCCTTTTATGAGT113890.15867564560813704No Hit
CTGCTCTGATGAAATCACTAATAGGAAGTGCCGTCAGAAGCGATAACTGA113560.15821587773518345No Hit
GGGGATGTAGCTCAGTGAAGATCGGAAGAGCACACGTCTGAACTCCAGTC113160.15765858334372457Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
TCCCACATGGTCTAGCGGTTAGGATTCCTGGTAGATCGGAAGAGCACACG111920.15593097073020196No Hit
TCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAGATCG111440.15526221746045124No Hit
GGAATACCGGGTGCTGTAGGCTTAGATCGGAAGAGCACACGTCTGAACTC100180.13957438034088304Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
GCATGGGTGGTTCAGTGGTAGAATTCTCGCCAGATCGGAAGAGCACACGT99510.13864091223518937No Hit
TAGCTTATCAGACTGATGTTGACAAGATCGGAAGAGCACACGTCTGAACT94790.13206483841597427Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
TGAGGTAGTAGATTGTATAGTCAGATCGGAAGAGCACACGTCTGAACTCC94770.13203697369640133Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
ACATTGATCATCGACACTTCGAACGCACTTGCGGCCCCGGGTTCCTCCCG93210.12986352556971162No Hit
GGCTGGTCCGAAGGTAGTGAGTTATCTCAATTAGATCGGAAGAGCACACG92220.12848422195085082No Hit
TTGAATGATGACTTTAATTGTCGGATACCCCTTCACTCCTTTTATGAGTG92010.1281916423953349No Hit
CCTCACTGATGAGTACGTTCTGACTTTCGTTCTTCTGAGTTTGCTGAAGC91250.12713278305156298No Hit
CATGGATGTGCTCTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAT89190.1242627169355496TruSeq Adapter, Index 1 (100% over 35bp)
TGAGGTAGTAGTTTGTGCTGTCAGATCGGAAGAGCACACGTCTGAACTCC87950.122535104322027Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
GGATGGTCCGATGGTAGTGGGTTATCAGAACTAGATCGGAAGAGCACACG87510.12192208049142218No Hit
TCGTACGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAAC87150.12142051553910918No Hit
CTGCGATGATGGCATTTCTTAGGACACCTTTGGATTAATAATGAAAACAA86110.11997155012131602No Hit
GTCTGGTCCGATGGTAGTGGGTTATCAGAACTAGATCGGAAGAGCACACG84230.11735226648145916No Hit
AATACATGATGATCTCAATCCAACTTGAACTCTCTCACTGATTACTTGAT81960.11418962080992988No Hit
TGGAAGACTAGTGATTTTGTTGTTAGATCGGAAGAGCACACGTCTGAACT81140.11304716730743912Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
GGCTGGTCCGATGGTAGTGGGTTATCAGAACTCAGATCGGAAGAGCACAC80090.11158426952985949No Hit
ATTGATCATCGACACTTCGAACGCACTTGCGGCCCCGGGTTCCTCCCGGG76960.10722344091669354No Hit
GGCTCTGTAGCTCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACATG76250.10623424337185398TruSeq Adapter, Index 15 (100% over 36bp)
GATGGGAGACCGCCTGGGAATACCGGGTGCTGTAGGCTTTAGATCGGAAG74940.10440910423982608No Hit
AGTGATGATGACCCCAGGTAACTCTGAGTGTGTCGCTGATGCCATCACCG74580.10390753928751306No Hit
TAGCTTATCAGACTGATGTTGAAAGATCGGAAGAGCACACGTCTGAACTC74170.10333631253626767Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
TGTTTGTGATGACTTACATGGAATCTCGTTCGGCTGATGACTTGCTGTTG74050.10316912421883No Hit
TGCCTCTGATGAAGCCTGTGTTGGTAGGGACATCTGAGAGTAATGATGAA72790.10141364688573445No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.8)