FastQCFastQC Report
Tue 16 Jul 2019
arabidopsis_SRR089618.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filenamearabidopsis_SRR089618.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6728223
Sequences flagged as poor quality0
Sequence length36
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTGGATTGAAGGGAGCTCTACTGTAGGCACCATCA1566002.3275090614564946No Hit
GCACCAGTGGTCTAGTGGTAGAATAGTACCCTGTAG1290721.9183668555575522No Hit
AGTTACTAATTCATGATCTGGCCTGTAGGCACCATC894621.3296527181099675No Hit
CTGTAGGCACCATCAATCGTATGCCGTCTTCTGCTT567250.8430903672485291Illumina Single End Adapter 2 (95% over 21bp)
TGAAGCTGCCAGCATGATCTGGCTGTAGGCACCATC552040.8204841010769114No Hit
TTCCACAGCTTTCTTGAACTTCTGTAGGCACCATCA469040.6971231482666375No Hit
TGAAGCTGCCAGCATGATCTACTGTAGGCACCATCA422830.6284423093586524No Hit
TTTGGATTGAAGGGAGCTCTTCTGTAGGCACCATCA416380.6188558256764082No Hit
TTGGACTGAAGGGAGCTCCCTTCTGTAGGCACCATC288410.4286570168676038No Hit
AGTTACTAATTCATGATCTGGCATCTGTAGGCACCA219440.3261485239118858No Hit
GCACCAGTGGTCTAGTGGTAGAATACTGTAGGCACC204350.3037206109250541No Hit
TTGAAAGTGACTACATCGGGGCTGTAGGCACCATCA187060.2780228895504801No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN171920.25552066273665425No Hit
TTCCACAGCTTTCTTGAACTGCTGTAGGCACCATCA166650.24768798537147177No Hit
CCAGTGGTCTAGTGGTAGAATAGTACCCTGTAGGCA165240.245592335450237No Hit
TGAAAGTGACTACATCGGGGTCTGTAGGCACCATCA143680.2135482132503634No Hit
TCCCAAATGTAGACAAAGCACTGTAGGCACCATCAA143210.2128496632766185No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA122350.1818459346546629No Hit
GCACCAGTTGTCTAGTGGTAGAATAGTACCCTGTAG119200.17716416355403203No Hit
GGCCGAGGGCACGTCTGCCTGGGCTGTAGGCACCAT117480.1746077679054336No Hit
GCACCAGTGGTCTAGTGGTAGAATAGTACTGTAGGC110350.16401061617606907No Hit
CAGCCAAGGATGACTTGCCGACTGTAGGCACCATCA98790.1468292593750237No Hit
TAGACCATTTGTGAGAAGGGACTGTAGGCACCATCA98360.14619016046287409No Hit
GCACCAGTGGTCTAGTGGTAGAATAGTACCCCTGTA94410.14031936813033694No Hit
GCACCAGTGGTCTAGTGGTAGAATCTGTAGGCACCA88930.1321745726917791No Hit
TTGGACTGAAGGGAGCTCCCTCTGTAGGCACCATCA74810.11118834794863368No Hit
ATTGTATCATTAACTATTTCTTTCTGTAGGCACCAT73500.1092413256813872No Hit
GCACCAGTGGTCTAGTGGTAGAATAGTACCTGTAGG73340.10900352143500594No Hit
GCGGACTGCTCGAGCTGCCTGTAGGCACCATCAATC72620.1079334023262903No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.8)